Abstract. The aim of this paper is to describe and assess the energetic-metabolic activity of selected muscles of upper and lower extremities duringRotational Training (RT). The influence of RT on temperaturechanges in thebiceps and triceps brachii muscles as well as the quadricepsand biceps femorismuscles of healthy university students were verified, in addition to temperaturedifferences between the left and right side before and after RT. The study wasconducted on 18 subjects. RT was conducted in accordance with accepted formsof training methodology of collective fitness. An importantpart of RT was theperformance of circular and rotational movements. Thermograms taken beforeand immediately after RT focused on 4 regions of selected agonists and syner-gists in the upper and lower body. The camera used was the infrared hand-heldCEDIP Titanium 560M IR (USA), located at a distance of 10 m from the sub-ject. After RT, medianTsk(skin temperature) decreased in almost all of theexamined ROIs, except for the left and right Qf (the quadriceps femoris, rectusfemoris, and sartorius muscles) as well as the left Tb (triceps brachii) in femalesubjects. In male subjects, left and rightTskof Qf increased both for the leftand right Tb. Left-Right∆Tsk(temperature difference) was statistically signif-icant (p <0.05) for Of, Bf, Bb, Tb in female as well as male subjects. It wasconcluded that thermography is a non-invasive, safe, and low-cost method forthe recording of physiological response of the skin to RT.