Introduction: Introduction. Streptococcus pneumoniae is a Gram-positive coccus causing mainly otitis media, community-acquired pneumonia, or even an invasive infections as bacteraemia. Generally, it is not accepted as the agent responsible for urinary tract infection (UTI). The case report describes S. pneumoniae isolate from the urine of 63-year-old woman with clinical and bacteriological symptoms of UTI, isolated independently in two bacteriological urine analyses. Conclusion. Results: The urine analyses showed leucocyturia. No other infections or genitourinary disorders were found. The patient was treated with BISEPTOL (trimethoprim/sulfamethoxazole) at a dose of 160/800 mg, twice a day for five days. After the treatment, the urinalysis showed the absence of the microorganisms in bacteriological evaluation. Conclusions: S. pneumoniae is considered to be a rare pathogen of UTI in adults.