Background: Litsea cubeba, also known as mountain pepper, belongs to the Lauraceae family. It has been used as a traditional herbal medicine since approximately 600 A.D., for example, in traditional Chinese medicine to treat inflammation, headaches, and intoxication. Essential oil extracted from the fresh fruits of Litsea cubeba is a clear, oily liquid with various activities, including antimicrobial, antioxidant, anticancerous, anti-inflammatory, and anti-diabetic properties. L. cubeba fruit has demonstrated cytotoxic effects on human lung, liver, and oral cancer cells. It is also shown to inhibit tyrosinase and reduce melanin production in epidermal cells, leading to decreased discoloration and skin aging. Aim: The objective of this study is to provide a comprehensive report regarding the chemical composition and safety profiles, as well as the antioxidant and anti-tyrosinase activities, of Litsea cubeba essential oil and its formulations. Methods: Gas chromatography-mass spectrometry was used to determine chemical composition. Cytotoxicity against VERO cells was checked after 6h, 12h, 24h, or 48h of incubation using an MTT colorimetric assay. Then, fibroblasts CCD-1059Sk were used to evaluate the toxicity of samples after 24h incubation. Antioxidant activity was screened in CUPRAC, FRAP, MCA, DPPH, ABTS, and PBD assays, while in the anti-tyrosinase test, activity was expressed as standard equivalents of kojic acid (KAE). Results: The GC-MS analysis confirmed the authenticity of the purchased EO, and the same profile was obtained for the formulations. In the cytotoxicity study, it is evident that the increase in cytotoxicity of L. cubeba essential oil is time-dependent, with the highest toxicity observed after 48 hours of incubation. In addition, it was noted that L. cubeba essential oil showed higher cytotoxicity towards CCD-1059Sk than VERO. However, the formulations, both cream and liquid, showed low toxicity to CCD-1059Sk. The antioxidant and anti-tyrosinase activities of the essential oil and its formulations varied based on the preparation examined. The cream formulation displayed the lowest antioxidant and anti-tyrosinase activity, while the liquid formulation exhibited the highest activity. Conclusion: Liquid formulation with Litsea cubeba essential oil was shown to be a safe and effective product for possible cosmetic anti-pigmentation/anti-aging applications. Key words: Cytotoxicity, Antioxidant, Anti-tyrosinase, Cream, Liquid, Bio, Natural