Introduction Knowledge about cone beam computed tomography (CBCT) among dental personnel is paramount topic and being investigated in many countries all over the world. Objectives: The aim of this study was to assess and compare knowledge and attitudes of Jordanian and Polish dental interns towards CBCT. Material and methods This cross-sectional study was performed using a questionnaire composed of 17 questions related to CBCT. The questionnaire was distributed to 89 Jordanian and 103 Polish dental interns, and  test was applied for data analysis. Results The majority of dental interns (range, 55.2-94.3%) correctly answered questions related to CBCT knowledge, and showed positive attitudes towards CBCT. Polish dental interns had higher scores in most of the questions related to CBCT basic knowledge, and there was a statistically significant difference in various responses (p < 0.05). Conclusions Both Jordanian and Polish dental interns have a good level of knowledge and positive attitudes towards CBCT. To enhance knowledge and attitudes among dental interns, dental faculties should provide more courses related to CBCT. keywords: cone beam CT, knowledge, attitudes, dental interns, Jordanian, Polish