Ecological corridors are zones of natural vegetation, which connect with other vegetation strips to create migration routes for animals and plants. The aim of our study was to investigate the occurrence and relative abundance of Dermacentor reticulatus in various habitats of the ecological corridor of the Wieprz River in eastern Poland. Ticks were collected using the flagging method in seven sites within the ecological corridor of the Wieprz River, i.e., one of the longest uninterrupted vegetation strips in eastern Poland. The presence of D. reticulatus adults was confirmed in each of the examined sites. The autumn peak of tick activity dominated in most plots. During this period, on average up to 309.7 individuals were collected within 30-min. The results of our study show that, due to the high abundance of local D. reticulatus populations, the habitats located in the ecological corridor of the Wieprz River can be regarded as preferred habitats of this tick species.